Statistik Volume Ekspor Buah-Buahan Indonesia Pretreatment methods for an effective conversion of oil palm biomass into sugars and high-value chemicals. The optimum reducing sugar yield can produce biofuel by the saccharification process. This result indicated that the enzymatic hydrolysis operating condition improved the reducing sugar yield from pineapple leaf waste. The model indicated that the optimum operating condition of enzymatic hydrolysis is 7 mL of cellulase enzyme at 55 oC to produce 96,673 mg/L reducing sugar. Determination of reducing sugar yield had been carried out using the Dubois method and HPLC. The sample was incubated in an incubator shaker at 155 rpm at 45, 55, and 60 oC for 72 hours. Delignified pineapple leaf waste has been enzymatic hydrolyzed using cellulase enzyme (6 mL, 7 mL, and 8 mL) to produce reducing sugar. Delignification process using 2% w/v NaOH solution at 87 oC for 60 minutes has been carried out to remove lignin and hemicellulose structure so reducing sugar could be produced. Dried pineapple leaf waste pretreated with a grinder to break its complex structure. Pineapple leaf waste's complex structure contains a bundle of packed fiber that makes it hard to remove lignin and hemicellulose structure, so challenging to produce reducing sugar. Pineapple leaf waste is one agricultural waste that has high cellulose content.
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